The BioTeZ Peroxidase Labeling Kit is intended for the preparation of Poly-Peroxidase labels using highly activated Poly-HRP (Horseradish Peroxidase). Poly-Peroxidase labels are characterized by high measurement signal amplification.
The pre-activated and polymerized Peroxidase contained in the kit can be used immediately for the coupling reaction with target molecules through the reaction with primary amino groups.
The kit can be applied for the production of Poly-Peroxidase conjugates of antibodies and other proteins. The coupling reaction takes place at primary amino groups on the antibody/protein using Pre-activates Poly-HRP, which form stable amide bonds. Generally, proteins have a sufficient number of amino groups for coupling, both N-terminal and lysine residues.
The labeling aliquotes are designed for a standard coupling of 1 mg antibody (immunoglobulins, such as IgG). For the labeling of other proteins, the amount to be used must be calculated accordingly.
BioTeZ Peroxidase Labeling Kit includes all necessary reagents and clean-up columns for the purification of the coupling product. All reagents are scheduled for 3 separate labeling reactions.
- Easy to conduct
- Reliable conjugation
- Poly-HRP provides strong signals and low NSB
LINK TO THE DATA SHEET
