The procedure is fast and simple with an average cell to sample purification time of less than 2 hours. Each kit provides sufficient reagents for preparing 10 extracts for an enrichment of HtrA1 about 200-300 ng per extract. Only now it is possible to determine the HtrA1 isoforms (Wester Blot, SDS-PAGE), the concentration (ELISAs) and activity of high diluted HtrA1.
Application
This kit provides a highly sensitive and straightforward, affinity-based procedure of extraction and enrichment of even low concentrated human HtA3 from cell culture e.g. mammalian cells and tissues e.g. placenta up to volumes of 1.4 ml. The procedure is fast and simple with an average cell to sample purification time of less than 2 hours. Each kit provides sufficient reagents for preparing 10 extracts for an enrichment of HtrA3 . Only now it is possible to determine the HtrA3 isoforms (Wester Blot, SDS-PAGE), the concentration (ELISAs) and activity of high diluted HtrA3.
Preservative
If applicable mentioned in the product data sheet
Storage
6 month
Storage Shipping Temp Min
ice pack
Storage Shipping Temp Max
ice pack
Storage Long Term
2-8°C
Principle
specific Affinity chromatography
